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Effect of anti‐apoptotic genes and peptide inhibitors on cytoplasmic acidification during apoptosis
Author(s) -
Pardhasaradhi B.V.V,
Khar Ashok,
Srinivas Usha K
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00665-0
Subject(s) - apoptosis , cytoplasm , transfection , intracellular , microbiology and biotechnology , programmed cell death , peptide , chemistry , biology , gene , biochemistry
Cytoplasmic acidification has been shown to occur during the apoptotic process of cell death although its relation with other events in the process are not yet clear. AK‐5 tumor cells have been shown to undergo apoptosis upon treatment with stimuli like dexamethasone (1 μM) or with serum from animals that reject AK‐5 tumor. The current study was designed to measure the extent of cytoplasmic acidification during apoptosis in AK‐5 cells and to study the effect of antiapopoptic genes and peptide inhibitors on cytoplasmic acidification. Our results show that AK‐5 cells when triggered into apoptosis show intracellular acidification by about 0.2 pH units and this is prevented when cells are treated with peptide inhibitors. In addition cytoplasmic acidification does not occur when AK‐5 cells are transfected with anti‐apoptotic genes Nedd‐2 A.S, Crm A or bcl‐2 which inhibit apoptosis.