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Cell‐cycle‐dependent phosphorylation of the basal transcription factor RAP74
Author(s) -
Yonaha Masatomo,
Tsuchiya Terumasa,
Yasukochi Yukio
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00642-x
Subject(s) - transcription factor , microbiology and biotechnology , phosphorylation , basal (medicine) , chemistry , cell cycle , cell , biology , biochemistry , endocrinology , gene , insulin
In this report, cell‐cycle‐dependent effects of TFIID on other basal transcription factors were investigated. We purified TFIID fractions from HeLa cells synchronized in the S/G2 phases and in early G1 phase, and show that RAP74 is phosphorylated more highly by the S/G2 phase TFIID fraction than by the early G1 phase TFIID fraction. Further analyses using deletion mutants of RAP74 revealed that amino acid residues 206–256 are phosphorylated by the TFIID fraction. Reconstitution of in vitro transcription activity indicates that the cell‐cycle‐dependent phosphorylation of RAP74 increases TFIIF transcription activity.