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Centrifugation does not alter spatial distribution of ‘BEP4’ mRNA in paracentrotus lividus EGG
Author(s) -
Costa Caterina,
Rinaldi Anna Maria,
Romancino Daniele P,
Cavalcante Carmela,
Vizzini Aiti,
Di Carlo Marta
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00641-8
Subject(s) - paracentrotus lividus , sucrose gradient , biology , centrifugation , colchicine , messenger rna , microbiology and biotechnology , biochemistry , sea urchin , gene , genetics , enzyme
Paracentrotus lividus unfertilized eggs were centrifuged in a sucrose gradient, so to split each into two parts: a nucleated light fragment and an anucleated heavy fragment. Northern blot analyses utilizing a bep4 probe as animal marker and H2A histone gene and 12S‐mit RNA as controls indicate that the eggs are elongated along the animal‐vegetal axis during centrifugation and thereafter split into an animal and a vegetal half. Treatment of the eggs with colchicine before centrifugation abolishes the animal localization of bep4 mRNA.