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Metabolic analysis of S. cerevisiae strains engineered for malolactic fermentation
Author(s) -
Bony M,
Bidart F,
Camarasa C,
Ansanay V,
Dulau L,
Barre P,
Dequin S
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00637-6
Subject(s) - malolactic fermentation , schizosaccharomyces pombe , lactococcus lactis , yeast , biochemistry , schizosaccharomyces , malic enzyme , biology , saccharomyces cerevisiae , fermentation , enzyme , chemistry , genetics , lactic acid , bacteria , dehydrogenase
A complete malolactic fermentation was achieved using Saccharomyces cerevisiae strains coexpressing the genes mleS and mae1 coding for the Lactococcus lactis malolactic enzyme and the Schizosaccharomyces pombe malate permease under the control of yeast promoters. The expression level of mae1 greatly influences the kinetics of the reaction by controlling the rate of malate uptake meanwhile a high expression level of mleS induces a partial consumption of malate derived from glucose by the malolactic enzyme. A strain expressing several copies of mae1 and one copy of mleS degrades 3 g/l of malate almost exclusively through the malolactic pathway in 4 days under enological conditions, without metabolic side effects.