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Dramatic enhancement of the specific expression of the heart‐type fatty acid binding protein in rat brown adipose tissue by cold exposure
Author(s) -
Daikoku Takiko,
Shinohara Yasuo,
Shima Atsushi,
Yamazaki Naoshi,
Terada Hiroshi
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00619-4
Subject(s) - brown adipose tissue , white adipose tissue , fatty acid binding protein , medicine , endocrinology , adipose tissue , western blot , thermogenin , biology , uncoupling protein , fatty acid , prdm16 , chemistry , biochemistry , gene
To understand the difference in energy metabolisms in brown (BAT) and white (WAT) adipose tissues, we examined the steady‐state transcript levels of the heart‐type and adipose‐type fatty acid binding proteins (H‐FABP and A‐FABP, respectively) by Northern blot analysis. The transcript of H‐FABP in rat BAT was increased about 100‐fold by cold exposure, whereas that in WAT was negligible, and was increased only slightly by cold exposure. The transcript of A‐FABP was observed in both BAT and WAT, the level being slightly greater in WAT. However, its transcript level was not affected by cold exposure in either adipose tissue. In addition, on treatment with norepinephrine (NE), transcript level of H‐FABP was elevated markedly but that of A‐FABP was not changed in rat brown adipocytes. Therefore, the stimulatory effect of cold exposure on the transcript of H‐FABP in BAT was concluded to be mediated by NE, like that of the uncoupling protein (UCP). Thus, the expressions of H‐FABP and UCP may be controlled by the same mechanism.