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Expressional downregulation of neuronal‐type NO synthase I in guinea pig skeletal muscle in response to bacterial lipopolysaccharide
Author(s) -
Gath Ingolf,
Gödtel-Armbrust Ute,
Förstermann Ulrich
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00615-7
Subject(s) - downregulation and upregulation , skeletal muscle , nitric oxide synthase , lipopolysaccharide , guinea pig , endocrinology , medicine , biology , messenger rna , chemistry , nitric oxide , biochemistry , gene
We have investigated the expression of neuronal‐type NO synthase I (NOS I) and inducible‐type NOS II in guinea pig skeletal muscle (diaphragm). Expression of NOS I mRNA and protein was highest in muscle of specific pathogen‐free animals, lower in normally bred animals, and lowest in lipopolysaccharide (LPS)‐treated animals. NOS II mRNA and protein levels were highest in muscle of LPS‐treated animals. Elevated NOS activity in muscle from LPS‐treated animals was less susceptible to the NOS I‐selective inhibitor N G ‐nitro‐ l ‐arginine. Expressional downregulation of NOS I in sepsis may have implications for contractile function of skeletal muscle.