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Direct action of nitric oxide on osteoblastic differentiation
Author(s) -
Hikiji Hisako,
Shin Wee Soo,
Oida Shinichiro,
Takato Tuyoshi,
Koizumi Toshiyuki,
Toyo-oka Teruhiko
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00597-8
Subject(s) - sodium nitroprusside , nitric oxide , alkaline phosphatase , osteocalcin , nitric oxide synthase , chemistry , tumor necrosis factor alpha , endocrinology , medicine , cytokine , gene expression , microbiology and biotechnology , enzyme , biochemistry , biology , gene
The effect of nitric oxide (NO) on osteoblastic differentiation was examined in cultured mouse osteoblasts. Interleukin‐1β and tumor necrosis factor‐α expressed inducible NO synthase gene with little effect on constitutive NO synthase gene. These cytokines increased NO production, which was inhibited by l ‐NMMA pretreatment, and decreased alkaline phosphatase (AIPase) activity, which was not restored by l ‐NMMA. Furthermore, NO donors, sodium nitroprusside and NONOate dose‐dependently elevated AIPase activity and expression of osteocalcin gene. These results suggest that NO directly facilitates osteoblastic differentiation and the cytokine‐induced inhibition of AIPase activity is mediated via mechanism other than NO.