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Iron triggers a rapid induction of ascorbate peroxidase gene expression in Brassica napus
Author(s) -
Vansuyt Gérard,
Lopez Félicie,
Inzé Dirk,
Briat Jean-Francois,
Fourcroy Pierre
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00587-5
Subject(s) - peroxidase , biochemistry , hydrogen peroxide , gene expression , chemistry , ascorbic acid , ferritin , oxidative stress , gpx3 , cysteine , heme , glutathione peroxidase , gene , enzyme , glutathione , food science
In plants, only ferritin gene expression has been reported to be iron‐dependent. Here it is demonstrated that an iron overload of Brassica napus seedlings causes a large and rapid accumulation of ascorbate peroxidase transcripts, a plant‐specific hydrogen peroxide‐scavenging enzyme. This result documents a novel link between iron metabolism and oxidative stress. The ascorbate peroxidase mRNA abundance was not modified by reducing agents like N ‐acetyl cysteine, glutathione and ascorbate or by pro‐oxidants such as hydrogen peroxide or diamide. Furthermore, the iron‐induced ascorbate peroxidase mRNA accumulation was not antagonized by N ‐acetyl cysteine. Abscisic acid had no effect on the ascorbate peroxidase gene expression. Taken together these results suggest that iron‐mediated expression of ascorbate peroxidase gene occurs through a signal transduction pathway apparently different from those already described for plant genes responsive to oxidative stress.