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AMP‐activated protein kinase isoenzyme family: subunit structure and chromosomal location
Author(s) -
Stapleton David,
Woollatt Erica,
Mitchelhill Ken I,
Nicholl Jillian K,
Fernandez C.Shamala,
Michell Belinda J,
Witters Lee A,
Power David A,
Sutherland Grant R,
Kemp Bruce E
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00569-3
Subject(s) - ampk , protein subunit , gene isoform , protein kinase a , amp activated protein kinase , gamma subunit , isozyme , microbiology and biotechnology , chemistry , biochemistry , gene , biology , kinase , enzyme
The AMP‐activated protein kinase (AMPK) consists of catalytic α and non‐catalytic, β and γ (38 kDa) subunits and is responsible for acting as a metabolic sensor for AMP levels. There are multiple genes for each subunit and we find that rat liver AMPK‐α2 isoform catalytic subunit is associated with β1 and γ1 and not with β2 or γ2 subunit isoforms. The β1 and γ1 isoforms are also subunits of the α1 isoform. The sequence of cloned human AMPK‐β1 is 95% identical in amino acid sequence with rat β1. Human chromosomal localizations were determined for AMPK‐α1 (5p11‐p14), AMPK‐β1 (12q24.1‐24.3) and AMPK‐γ1 (12q12‐q14), respectively.