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Modulation of cholinergic locus expression by glucocorticoids and retinoic acid is cell‐type specific
Author(s) -
Berse Brygida,
Blusztajn Jan Krzysztof
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00568-1
Subject(s) - vesicular acetylcholine transporter , choline acetyltransferase , retinoic acid , cholinergic , medicine , glucocorticoid , endocrinology , biology , acetylcholine , cholinergic neuron , microbiology and biotechnology , cell culture , genetics
Modulation of mRNA expression of choline acetyltransferase (ChAT) and the vesicular acetylcholine transporter (VAChT) by the glucocorticoid dexamethasone and by retinoic acid was examined in two neuronal cell lines: basal forebrain‐derived SN56 and pheochromocytoma PC12. Dexamethasone up‐regulated ChAT and VAChT in SN56 cells, while it had inhibitory effects on these genes in PC12 cells. Retinoic acid stimulated the cholinergic markers in both cell types, but in SN56 cells its effect was partially additive with that of dexamethasone, whereas it was much smaller and abrogated by dexamethasone in PC12 cells. Acetylcholine content correlated with these mRNA changes. The presence of a glucocorticoid response element consensus sequence in the VAChT/ChAT gene locus suggests direct transcriptional regulation by glucocorticoids.