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Vp165 and GLUT4 share similar vesicle pools along their trafficking pathways in rat adipose cells
Author(s) -
Malide Daniela,
St-Denis Jean-François,
Keller Susanna R.,
Cushman Samuel W.
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00563-2
Subject(s) - glut4 , microbiology and biotechnology , vesicle , wortmannin , vesicle fusion , endocytic cycle , adipose tissue , chemistry , biology , glucose transporter , biochemistry , endocytosis , insulin , membrane , signal transduction , cell , endocrinology , synaptic vesicle , pi3k/akt/mtor pathway
vp165 (or gp160) is an aminopeptidase that has been identified as one of the major proteins of the GLUT4‐containing vesicles. In the present study we have determined the degree of co‐localization between vp165 and GLUT4 in rat adipose cells and used perturbation by wortmannin to assess the exocytic and endocytic steps along the translocation and recycling pathways of GLUT4 in the absence and presence of insulin. Western blots of subcellular membrane fractions demonstrate very similar distributions of vp165 and GLUT4. Confocal microscopy of whole cells provides direct evidence that these proteins share the same vesicle populations moving both towards and from the plasma membrane. These data are consistent with the presence of a distinct insulin‐sensitive compartment that sequesters both GLUT4 and vp165 and suggest similar trafficking routes through the recycling compartments.