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Structural features of the gene encoding human muscle type carnitine palmitoyltransferase I 1
Author(s) -
Yamazaki Naoshi,
Yamanaka Yasuhisa,
Hashimoto Yoshiko,
Shinohara Yasuo,
Shima Atsushi,
Terada Hiroshi
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00561-9
Subject(s) - exon , gene , intron , complementary dna , exon trapping , coding region , biology , microbiology and biotechnology , tandem exon duplication , genomic dna , nucleic acid sequence , genetics , carnitine palmitoyltransferase i , alternative splicing , biochemistry , fatty acid , beta oxidation
We isolated a human muscle type of carnitine palmitoyltransferase I (CPTI‐M) genomic clone and determined its entire nucleotide sequence. By comparison of the nucleotide sequence of the genomic clone with that of cDNA, we determined the intron/exon junctions. For detection of the exon(s) in the 5′‐region of the CPTI‐M gene, we isolated cDNA clones corresponding to the 5′‐region of its transcript by 5′‐rapid amplification of cDNA ends (5′‐RACE method). Results showed two alternative exons, 1A and 1B, that do not encode amino acids in the 5′‐region of the human CPTI‐M gene. The gene encoding human CPTI‐M was found to consist of two 5′‐non‐coding exons, 18 coding exons and one 3′‐non‐coding exon spanning approximately 10 kbp. Furthermore, on analysis of the 5′‐flanking region, a putative gene encoding a ‘choline kinase homologue’ was found to be located only about 300 bp upstream from exon 1A of the human CPTI‐M gene. Comparison of the gene structure of human CPTI‐M with the reported partial gene structure of human liver type CPTI (CPTI‐L) showed that the intron insertion sites were completely conserved in these two genes.