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Molecular cloning of a diacylglycerol kinase isozyme predominantly expressed in rat retina
Author(s) -
Kohyama-Koganeya Ayako,
Watanabe Masahiko,
Hotta Yoshiki
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00526-7
Subject(s) - diacylglycerol kinase , isozyme , open reading frame , biology , complementary dna , molecular cloning , retina , homology (biology) , microbiology and biotechnology , cloning (programming) , protein kinase c , biochemistry , kinase , amino acid , enzyme , gene , peptide sequence , neuroscience , computer science , programming language
We have cloned and characterized a new diacylglycerol kinase (DGK) isozyme which is expressed in the retina and the brain of rat. The cDNA contains an open reading frame of 567 amino acid residues with a predicted protein of 64 kDa and shows very high homology to human DGKϵ. The new DGK isozyme contains two distinctive zinc‐finger structures and a putative catalytic domain. This DGK expressed predominantly in the inner and outer nuclear layers of retina. This expression pattern is different from those of the previously cloned DGKs including the human DGKϵ, suggesting that this DGK isozyme has potential importance in visual functions as was the case in Drosophila retinal cells.