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Molecular cloning and expression of a bovine endothelial inward rectifier potassium channel 1
Author(s) -
Forsyth Scott E,
Hoger Anne,
Hoger Jeff H
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00514-0
Subject(s) - inward rectifier potassium ion channel , complementary dna , xenopus , cdna library , microbiology and biotechnology , potassium channel , amino acid , peptide sequence , cloning (programming) , transmembrane protein , biology , extracellular , chemistry , gene , biochemistry , ion channel , biophysics , receptor , computer science , programming language
A 5.1 kb cDNA encoding an inward rectifier K + channel (BIK) was isolated from a bovine aortic endothelial cell library. The cDNA codes for a 427‐amino‐acid protein with two putative transmembrane regions. Sequence analysis reveals that BIK is a member of the Kir2.1 family of inward rectifier K + channels. Expression in Xenopus oocytes showed that BIK is a K + ‐specific strong inward rectifier channel that is sensitive to extracellular Ba 2+ , Cs + , and a variety of anti‐arrhythmic agents. Northern analysis revealed that endothelial cells express a 5.5 kb BIK mRNA that is sensitive to shear stress.