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Tyrosine: an inhibitor of LDL oxidation and endothelial cell cytotoxicity initiated by superoxide/nitric oxide radicals 1
Author(s) -
Kapiotis Stylianos,
Hermann Marcela,
Held Irmtraud,
Mühl Adolf,
Gmeiner Bernhard
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00513-9
Subject(s) - chemistry , radical , superoxide , tyrosine , nitric oxide , cytotoxicity , tbars , biochemistry , antioxidant , endothelial stem cell , lipid peroxidation , in vitro , organic chemistry , enzyme
Tyrosyl radicals can catalyze LDL oxidation. In addition to their LDL oxidizing ability, superoxide (O 2 ·− )/nitric oxide (NO · ) generate phenoxyl radicals when reacting with tyrosine. Therefore we tested if tyrosine can act as a pro‐oxidant in O 2 ·− /NO · ‐initiated LDL oxidation. When LDL was exposed to O 2 ·− /NO · , tyrosine exerted a strong inhibitory effect on O 2 ·− /NO · ‐initiated LDL oxidation as measured by TBARS formation and alteration in electrophoretic mobility of LDL. Tyrosine was also able to protect human endothelial cells from the cytotoxic effect of O 2 ·− /NO · . Because O 2 ·− /NO · can occur in vivo, the results may indicate that serum‐free tyrosine could act as an efficacious physiological antioxidant in case of O 2 ·− /NO · ‐initiated LDL oxidation and endothelial cell cytotoxicity.