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Differential involvement of caspases in apoptosis of myeloid leukemic cells induced by chemotherapy versus growth factor withdrawal
Author(s) -
Barge Renee M.Y,
Willemze Roel,
Vandenabeele Peter,
Fiers Walter,
Beyaert Rudi
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00507-3
Subject(s) - apoptosis , caspase , chemotherapy , programmed cell death , cancer research , intrinsic apoptosis , myeloid leukemia , cell culture , biology , microbiology and biotechnology , biochemistry , genetics
To assess the potential involvement of the caspase family in the IL‐3‐dependent murine myeloid leukemic cell line 32D, we studied the effect of bcl‐2, crmA and three synthetic caspase inhibitors on apoptosis induced by chemotherapy or IL‐3 withdrawal. Apoptosis induced by IL‐3 deprivation or by ActD appears to be mediated by a crmA‐insensitive pathway. Cell death by IL‐3 withdrawal is inhibited by the caspase‐inhibitor ZVAD‐fmk, but not DEVD‐fmk or YVAD‐cmk. In contrast, DEVD‐fmk as well as ZVAD‐fmk protect 32D cells from ActD‐induced apoptosis. These results indicate that different caspases are involved in apoptosis induced by growth factor withdrawal and by chemotherapy.