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Stimulatory effects of parathyroid hormone and 1,25‐dihydroxyvitamin D 3 on insulin‐like growth factor‐binding protein‐5 mRNA expression in osteoblastic UMR‐106 cells: the difference between transient and continuous treatments
Author(s) -
Nasu Masamichi,
Sugimoto Toshitsugu,
Chihara Kazuo
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00484-5
Subject(s) - parathyroid hormone , endocrinology , medicine , messenger rna , insulin , hormone , chemistry , growth factor , biology , receptor , calcium , gene , biochemistry
The transient treatment with parathyroid hormone (PTH) for 12 h, followed by its removal for 36 h, stimulated insulin‐like growth factor‐binding protein (IGFBP)‐5 mRNA expression more strongly than the continuous treatment for 48 h in osteoblastic UMR‐106 cells. The transient but not continuous treatment with A23187 also stimulated it. In contrast, 1,25‐dihydroxyvitamin D 3 stimulated it, irrespective of the treatment design. IGFBP‐5 stimulated type‐1 procollagen mRNA expression. The present study first indicated that the transient treatment with PTH more effectively stimulated IGFBP‐5 mRNA expression than its continuous treatment partly via an increase in intracellular calcium and suggested that IGFBP‐5 might be involved in the anabolic action of PTH in bone.