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Nitric oxide protects endothelial cells from tumor necrosis factor‐α‐mediated cytotoxicity: possible involvement of cyclic GMP
Author(s) -
Polte Tobias,
Oberle Stefanie,
Schröder Henning
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00480-8
Subject(s) - cytoprotection , nitric oxide , tumor necrosis factor alpha , cytotoxicity , incubation , chemistry , endothelial stem cell , microbiology and biotechnology , viability assay , soluble guanylyl cyclase , necrosis , pharmacology , biochemistry , in vitro , cyclic gmp , apoptosis , biology , immunology , medicine , organic chemistry
In cultured endothelial cells, incubation with TNF‐α (50 ng/ml) for 48 h markedly reduced viability of endothelial cells. A 6 h preincubation with Sper/NO (0.03‐−1 μM) protected endothelial cells in a concentration‐dependent manner and increased viability by 63% of control. The NO scavenger PTIO (30 μM) completely abolished cytoprotection by Sper/NO. A cytoprotective effect comparable to Sper/NO was observed when preincubating the cells with 8‐bromo cyclic GMP (1–10 μM). Moreover, no protection by Sper/NO occurred in the presence of ODQ (0.1 μM), a selective inhibitor of soluble guanylyl cyclase. Our results demonstrate that NO produces a long‐term endothelial protection against cellular injury by TNF‐α, presumably via a cyclic GMP‐dependent pathway.