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Endogenously produced peroxynitrite induces the oxidation of mitochondrial and nuclear proteins in immunostimulated macrophages
Author(s) -
Szabó Csaba,
O'Connor Michael,
Salzman Andrew L
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00466-3
Subject(s) - peroxynitrite , chemistry , lipopolysaccharide , nitric oxide , peroxynitrous acid , mitochondrion , nitric oxide synthase , biochemistry , superoxide dismutase , superoxide , microbiology and biotechnology , oxidative stress , biology , enzyme , immunology , organic chemistry
Here we investigated the role of endogenous nitric oxide (NO) and peroxynitrite in the process of protein oxidation (as measured by the detection of 2,4‐dinitrophenylhydrazine‐reactive carbonyls) in immunostimulated macrophages. Immunostimulation of the macrophages by bacterial lipopolysaccharide and gamma‐interferon (LPS/IFNγ) resulted in a marked increase in the oxidation of a large number of mitochondrial and nuclear proteins. The inhibitor of NO synthase, N G ‐methyl‐ l ‐arginine (3 mM), and the cell‐permeable superoxide dismutase mimetic Mn(III)tetrakis(4‐benzoic acid)porphyrin (300 μM) both reduced the extent of protein oxidation in response to LPS/IFNγ. These results support the view that endogenously produced peroxynitrite induces protein oxidation in the mitochondria and nucleus of immunostimulated macrophages.