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Microinjected cDNA encoding JAK2 protein‐tyrosine kinase induces DNA synthesis in NIH 3T3 cells 1
Author(s) -
Smith Mark R.,
Duhé Roy J.,
Liu Ya-lun,
Farrar William L.
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00456-0
Subject(s) - dna synthesis , microbiology and biotechnology , plasmid , complementary dna , 3t3 cells , microinjection , biology , dna , transfection , gene , biochemistry
Microinjection of expression plasmids encoding either JAK2 or hyperactive (NΔ661)rJAK2 into serum‐starved NIH 3T3 cells resulted in 20–30‐fold induction of DNA synthesis. Control microinjections of buffer or parental pcDNA3 vector resulted in only 3–5‐fold induction of DNA synthesis. Induction of DNA synthesis was blocked when plasmid encoding JAK2 was microinjected in the presence of the JAK2‐selective inhibitor AG‐490, whereas AG‐490 did not block DNA synthesis induced by microinjected plasmid encoding (NΔ661)rJAK2. The ability of JAK2 to initiate the G o /S cell cycle transition is comparable to that of other proto‐oncogenes, and supports a mechanistic role for overexpressed Janus kinases in carcinogenesis.