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Addition of G418 and other aminoglycoside antibiotics to mammalian cells results in the release of GPI‐anchored proteins
Author(s) -
Küng Matthias,
Stadelmann Barbara,
Brodbeck Urs,
Bütikofer Peter
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00452-3
Subject(s) - neomycin , aminoglycoside , transfection , antibiotics , alkaline phosphatase , microbiology and biotechnology , cell culture , cell , chemistry , plasmid , biology , gene , biochemistry , enzyme , genetics
Resistance to the neomycin analogue G418 forms the basis of a dominant marker selection system for mammalian cells transfected with the bacterial neomycin gene. We found that COS‐1 cells stably transfected with the neomycin resistance gene had a greater than 50% reduction in cell‐associated glycosylphosphatidylinositol (GPI)‐anchored alkaline phosphatase (AP). A similarly reduced amount of AP was also observed in wild‐type COS‐1 cells incubated in the presence of G418 or other aminoglycoside antibiotics. The AP was released from cells into the culture supernatant in its GPI‐anchored form. Our data suggest that the G418‐induced reduction of AP involves a vesiculation process of COS‐1 cells.