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Lipopolysaccharide interaction with hemolin, an insect member of the Ig‐superfamily
Author(s) -
Daffre Sirlei,
Faye Ingrid
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00397-9
Subject(s) - hemolymph , lipopolysaccharide , biology , escherichia coli , insect , lipid a , microbiology and biotechnology , biochemistry , ecology , immunology , gene
This study is an attempt to reach some understanding of how insects recognize intruding microorganisms and foreign entities while executing an immune response. We used lipopolysaccharide (LPS) from Escherichia coli , bound to a radiolabeled iodinated crosslinker, to identify hemolymph proteins from the Hyalophora cecropia moth that have the capacity to bind LPS. High amounts of radioactivity were conferred to hemolin, an immunoglobulin and NCAM‐related protein, the concentration of which increases in insect hemolymph upon bacterial infection. We could demonstrate a concentration‐dependant binding of hemolin to LPS. In addition we could show that Lipid A can compete for this binding, whereas KDO has no effect, indicating that hemolin interacts specifically with the Lipid A moiety of LPS.