Premium
Transcriptional activity and constitutive nuclear localization of the ETS protein Elf‐1
Author(s) -
Bredemeier-Ernst Isa,
Nordheim Alfred,
Janknecht Ralf
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00387-6
Subject(s) - ets transcription factor family , nuclear localization sequence , transcription factor , transcription (linguistics) , fusion protein , psychological repression , nuclear protein , gene , microbiology and biotechnology , nls , dna binding domain , biology , amino acid , nuclear transport , genetics , cell nucleus , gene expression , recombinant dna , philosophy , linguistics
Elf‐1 is a lymphoid‐specific transcription factor that belongs to the ETS protein family. It can bind to DNA target sequences within a variety of cytokine genes. We demonstrate that Elf‐1 is constitutively localized in the nucleus which is dependent on the presence of amino acids 86–265. Analysis of Gal4‐Elf‐1 fusion proteins revealed that the N‐terminal 86 amino acids of Elf‐1 contain a transcriptional activation domain, the activity of which is attenuated by an internal repression domain. Furthermore, Elf‐1 interacts specifically with the E74 target sequence and can stimulate transcription driven by the E74 site independent of mitogenic signaling. Thus, Elf‐1 is able to stimulate gene transcription which may be required for the development and activity of lymphocytes.