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Stimulation of human keratinocyte growth by alginate oligosaccharides, a possible co‐factor for epidermal growth factor in cell culture
Author(s) -
Kawada Akira,
Hiura Nozomi,
Shiraiwa Masakazu,
Tajima Shingo,
Hiruma Masataro,
Hara Kenji,
Ishibashi Akira,
Takahara Hidenari
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00386-4
Subject(s) - epidermal growth factor , stimulation , keratinocyte growth factor , keratinocyte , growth factor , microbiology and biotechnology , cell culture , chemistry , factor (programming language) , cell growth , biology , biochemistry , in vitro , endocrinology , computer science , receptor , genetics , programming language
Oligosaccharides, involved in regulation of plant developmental and defensive processes, were tested to determine their ability to enhance proliferation of human keratinocytes. A mixture of alginate oligosaccharides remarkably stimulated keratinocyte growth and [ 3 H]thymidine uptake in the presence of epidermal growth factor (EGF). The activity was comparable to bovine pituitary extract, a common complement in keratinocyte culture, and additive on BPE‐induced stimulation. The most effective oligosaccharide in the mixture was identified and its chemical structure was determined. These findings demonstrate a novel activity of alginate oligosaccharide(s) in keratinocyte growth and suggest a possible co‐factor for EGF‐dependent stimulation in medium for keratinocytes.