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Induction of glutathione synthetase by 1,10‐phenanthroline
Author(s) -
Sun Yi
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00380-3
Subject(s) - microbiology and biotechnology , glutathione , apoptosis , phenanthroline , transfection , gene , biology , oncogene , dna fragmentation , glutathione synthetase , chemistry , biochemistry , programmed cell death , enzyme , cell cycle , inorganic chemistry
The differential display (DD) was employed to identify the gene(s) responsible for 1,10‐phenanthroline (OP)‐induced apoptosis in murine tumor cells (Sun, Y., Bian, J., Wang, Y. and Jacobs, C. (1997) Oncogene 14, 385–393 [1]). An OP‐inducible gene was isolated which encodes mouse glutathione synthetase (GSS). The GSS mRNA level began to increase 6 h post OP treatment and remained at a high level thereafter up to 24 h tested. Induction of GSS was found not to be associated with p53 activation. No significant induction of DNA fragmentation was detected in two murine tumor lines upon GSS transfection. This is the first observation indicating that GSS is inducible rather specifically by a metal chelator and that induction of GSS, however, is not sufficient to induce apoptosis. It may merely reflect a cellular response to OP‐induced redox disturbance.