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Expression of a highly basic peroxidase gene in NaCl‐adapted tomato cell suspensions
Author(s) -
Medina Marı́a I.,
Botella Miguel A.,
Quesada Miguel A.,
Valpuesta Victoriano
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00379-7
Subject(s) - peroxidase , gene expression , gene , in vitro , suspension culture , cell culture , plant cell , biology , suspension (topology) , genetically modified tomato , microbiology and biotechnology , salt (chemistry) , chemistry , botany , biochemistry , enzyme , genetics , genetically modified crops , transgene , mathematics , homotopy , pure mathematics
A tomato peroxidase gene, TPX2, that is only weakly expressed in the roots of young tomato seedlings is highly expressed in tomato suspension cells adapted to high external NaCl concentration. The protein encoded by this gene, with an isolectric point value of ≈9.6, is found in the culture medium of the growing cells. Our data suggest that the expression of TPX2 in the salt‐adapted cells is not the result of the elicitation imposed by the in vitro culture or the presence of high NaCl concentration in the medium.