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Function of the repeated sequence in the 3′ flanking region of the Escherichia coli rnpB gene on transcription termination and RNA processing
Author(s) -
Kim Semi,
Lee Younghoon
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00377-3
Subject(s) - terminator (solar) , rnase p , biology , transcription (linguistics) , genetics , gene , rna , termination factor , escherichia coli , coding region , microbiology and biotechnology , non coding rna , physics , ionosphere , linguistics , philosophy , astronomy
The 3′ flanking region of the Escherichia coli rnpB gene‐encoding M1 RNA, the RNA component of RNase P, contains a 113 bp repeated sequence. This sequence, successively reiterating 3.5 times, includes the region for intrinsic termination. In vivo termination of transcription occurs mostly at the first terminator (T1). Analysis of deletions at the 3′ flanking region revealed that the second terminator (T2) and third (T3) are functional in vivo and that the sequences preceding the region coding for an RNA‐terminator hairpin and U‐rich 3′ tail are essential for efficient termination. Transcripts terminating at T2 and T3 were also processed at the 3′ end in a manner similar to those terminating at T1.