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Activation of MAP kinase cascade induced by human pancreatic phospholipase A 2 in a human pancreatic cancer cell line
Author(s) -
Kinoshita Eiji,
Handa Noriko,
Hanada Keiji,
Kajiyama Goro,
Sugiyama Masanori
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00373-6
Subject(s) - mapk/erk pathway , phosphorylation , mapk cascade , pancreatic cancer , kinase , chemistry , mitogen activated protein kinase , microbiology and biotechnology , protein kinase a , cancer research , cell growth , biochemistry , biology , medicine , cancer
We have found that the growth of human pancreatic cancer cells MIAPaCa‐2, induced by human pancreatic phospholipase A 2 group I (hPLA 2 ‐I), is mediated via its specific receptor but not via its catalytic property. The present study showed that the activation of mitogen‐activated protein kinase (MAPK) cascade in MIAPaCa‐2 cells is induced by hPLA 2 ‐I: this digestive enzyme induced phosphorylation of MEK1/2, p44/42 MAPK and ATF‐2, and the phosphorylation in the MAPK cascade was inhibited after the cells were pre‐incubated with a selective inhibitor of MEK, PD98059. In addition, this inhibitor dose‐dependently blocked the hPLA 2 ‐I‐induced MIAPaCa‐2 proliferation, suggesting that activation of the MAPK cascade is essential for the hPLA 2 ‐I‐induced MIAPaCa‐2 proliferation.