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Recombinant human O 6 ‐alkylguanine‐DNA alkyltransferase induces conformational change in bound DNA
Author(s) -
Federwisch Matthias,
Hassiepen Ulrich,
Bender Kirsten,
Rajewsky Manfred F,
Wollmer Axel
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00370-0
Subject(s) - recombinant dna , dna , circular dichroism , chemistry , conformational change , microbiology and biotechnology , fluorescence , biophysics , biochemistry , biology , gene , physics , quantum mechanics
Circular dichroism, and steady‐state and time‐resolved fluorescence spectroscopy were used to compare the native recombinant human DNA repair protein O 6 ‐alkylguanine‐DNA alkyltransferase (AGT) with AGT bound to ds‐DNA. Contrary to fluorescence, analysis of the far‐UV CD spectra indicated a conformational change of AGT upon binding to DNA: its α‐helical content is increased by ∼12%. Analysis of near‐UV CD spectra revealed that DNA was also affected, probably being separated into single strands locally.