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Ap 3 A and Ap 4 A are primers for oligoadenylate synthesis catalyzed by interferon‐inducible 2–5A synthetase 1
Author(s) -
Turpaev Kyril,
Hartmann Rune,
Kisselev Lev,
Justesen Just
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00365-7
Subject(s) - interferon , primer (cosmetics) , enzyme , adenylylation , microbiology and biotechnology , biology , aminoacyl trna synthetase , catalysis , biochemistry , chemistry , biosynthesis , gene , transfer rna , rna , virology , organic chemistry
The biological role of Ap 3 A synthesized in cells by tryptophanyl‐tRNA synthetase (WRS) is unknown. Previously we have demonstrated that the cellular level of Ap 3 A significantly increases after interferon treatment. Here we show that the human 46 kDa 2‐5A synthetase efficiently utilizes Ap 3 A as a primer for oligoadenylate synthesis. The K m for Ap 3 A is several‐fold lower than for Ap 4 A and 100‐fold lower than for ATP. This implies that Ap 3 A might be a natural primer for the 2′‐adenylation reaction catalysed by 2‐5A synthetase. Since WRS and 2‐5A synthetase are both interferon‐inducible proteins, a new link between two interferon‐dependent enzymes is established.