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A new transglutaminase‐like from the ascidian Ciona intestinalis 1
Author(s) -
Cariello Lucio,
Ristoratore Filomena,
Zanetti Laura
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00342-6
Subject(s) - ciona intestinalis , tissue transglutaminase , complementary dna , open reading frame , ciona , cdna library , biology , peptide sequence , amino acid , biochemistry , enzyme , microbiology and biotechnology , gene
A cDNA clone encoding a transglutaminase (TGase) was isolated from a cDNA library prepared from the larval stage of Ciona intestinalis . The cDNA sequence has an open reading frame encoding a protein of 696 amino acids and is about 36% identical to 11 other TGase sequences. In addition, the critical residues thought to form the catalytic center are conserved. The Ciona TGase (CiTGase) has an extension of 39 amino acids in the NH 2 ‐terminal region similar to that reported for keratinocyte TGases. A phylogenetic analysis among other types of TGases demonstrated that CiTGase represents a new type of the enzyme.