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‘Green mice’ as a source of ubiquitous green cells
Author(s) -
Okabe Masaru,
Ikawa Masahito,
Kominami Katsuya,
Nakanishi Tomoko,
Nishimune Yoshitake
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00313-x
Subject(s) - green fluorescent protein , bioluminescence , transgene , fluorescence , complementary dna , microbiology and biotechnology , aequorea victoria , cell culture , biology , genetically modified mouse , mutant , chemistry , gene , biochemistry , genetics , physics , quantum mechanics
The green fluorescent protein (GFP) is responsible for the green bioluminescence of the jellyfish Aequorea victoria . Many classes of GFP mutants exist that display modified fluorescence spectra and an increased extinction coefficient. We produced transgenic mouse lines with an ‘enhanced’ GFP (EGFP) cDNA under the control of a chicken beta‐actin promoter and cytomegalovirus enhancer. All of the tissues from these transgenic lines, with the exception of erythrocytes and hair, were green under excitation light. The fluorescent nature of the cells from these transgenic mouse lines would facilitate their use in many kinds of cell transplantation experiments.