Premium
Interaction of the G‐protein G 11 α with receptors and phosphoinositidase C:
Author(s) -
Wise Alan,
Parenti Marco,
Milligan Graeme
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00300-1
Subject(s) - palmitoylation , receptor , inositol phosphate , transfection , g protein , agonist , stimulation , hek 293 cells , wild type , g protein coupled receptor , chemistry , mutant , inositol , biochemistry , microbiology and biotechnology , biology , endocrinology , gene , enzyme , cysteine
Wild‐type and palmitoylation defective mutants of the murine G protein G 11 α were transfected into HEK293 cells. Wild‐type G 11 α was membrane associated, Cys9Ser Cys10Ser G 11 α was present in the soluble fraction whilst both Cys9Ser G 11 α and Cys10Ser G 11 α were distributed between the fractions. Expression of the rat TRH receptor resulted in agonist stimulation of inositol phosphate accumulation. The degree of stimulation produced by TRH following co‐transfection of the palmitoylation‐resistant forms of G 11 α compared to the wild‐type protein correlated with the amount of membrane‐associated G protein.