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The effect of neuropeptides kyotorphin and neokyotorphin on proliferation of cultured brown preadipocytes
Author(s) -
Bronnikov Gennady,
Dolgacheva Ludmila,
Zhang Shi-Jin,
Galitovskaya Elena,
Kramarova Ludmila,
Zinchenko Valery
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00298-6
Subject(s) - norepinephrine , medicine , endocrinology , chemistry , receptor , stimulation , neuropeptide , biology , dopamine
Stimulation of DNA and protein synthesis in brown preadipocytes by 1 μM neokyotorphin in serum‐containing media was comparable with the effect of 1 μM norepinephrine. In serum‐free medium a decrease and a shift of the maximal effect to lower concentration of neokyotorphin were observed. Kyotorphin had no effect on cell proliferation in either medium; however, 0.01–1 μM kyotorphin inhibited the cell proliferation stimulated by 1 μM norepinephrine. Norepinephrine and both peptides stimulated comparable Ca 2+ rise in freshly isolated brown preadipocytes. The effects of neokyotorphin and norepinephrine were additive, whereas 0.03–0.3 μM kyotorphin blocked the action of 3 μM norepinephrine. The peptides did not affect the cAMP level in non‐stimulated or norepinephrine‐stimulated cultured cells. The effects of the peptides on the brown fat cell cultures indicate that peripheral tissue cells contain receptors for these neuropeptides.