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Distinct distal and proximal p53‐binding sites in the MCK promoter govern the transcriptional response to p53
Author(s) -
Jackson Paul,
Yardley Gina
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00283-4
Subject(s) - transcription (linguistics) , binding site , promoter , transcription factor , promoter activity , nucleotide , chemistry , response element , microbiology and biotechnology , biology , gene , biochemistry , gene expression , philosophy , linguistics
We have investigated the functional importance of nucleotide sequence differences between proximal (P‐) and distal (D‐) p53‐binding elements in the MCK promoter. P‐ and D‐elements normally co‐operate to permit synergistic promoter activation by p53. Interestingly, we find that P‐elements cannot co‐operate with each other. In contrast, co‐operation between D‐binding sites results in levels of p53‐induced transcription far higher than those obtained by co‐operation between P‐ and D‐elements. These studies imply that distinct D‐ and P‐p53‐binding sites in the MCK promoter may dictate the promoter response to p53.