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In vitro cytotoxic effects of tumor necrosis factor‐α in human breast cancer cells may be associated with increased glucose consumption
Author(s) -
Kaplan Ofer,
Ruı́z-Cabello Jesús,
Cohen Jack S
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00265-2
Subject(s) - tumor necrosis factor alpha , necrosis , cytotoxic t cell , in vivo , cytotoxicity , cancer cell , endocrinology , cancer research , chemistry , cell culture , biology , in vitro , medicine , pharmacology , cancer , biochemistry , microbiology and biotechnology , genetics
Tumor necrosis factor‐α inhibited growth of cultured MCF‐7 human breast cancer cells in a dose dependent manner. Tumor necrosis factor‐α also markedly increased glucose consumption, and its cytotoxicity was modified by glucose concentrations in the growth medium; higher glucose levels were associated with increased cell survival. However, when the cells were perfused in physiological conditions, very high levels of tumor necrosis factor‐α (200 ng/ml) in the perfusion solution had no inhibitory effects. Moreover, tumor necrosis factor‐α had no effects on 31 P nuclear magnetic resonance spectra of the perfused cells. In the traditional growth inhibition assays, cells are incubated for several days with a drug, a situation where their metabolism is altered due to the depletion of nutrients, the accumulation of toxic waste materials and pH changes. Perfusion experiments are more relevant to in vivo conditions, and may be used for studying metabolic processes and the mechanisms of action of therapeutic agents.