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Co‐localization of glucokinase with actin filaments
Author(s) -
Murata Tomiyasu,
Katagiri Hideki,
Ishihara Hisamitsu,
Shibasaki Yoshikazu,
Asano Tomoichiro,
Toyoda Yukiyasu,
Pekiner Bilgehan,
Pekiner Can,
Miwa Ichitomo,
Oka Yoshitomo
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00253-6
Subject(s) - glucokinase , cytoplasm , subcellular localization , microbiology and biotechnology , actin , islet , transfection , nucleus , biology , chemistry , biochemistry , endocrinology , insulin , gene
A portion of glucokinase appeared to be co‐localized with actin filaments in the cytoplasm of cultured rat hepatocytes incubated with 25 mM glucose. When liver‐ or islet‐type glucokinase was transiently expressed in COS‐7 cells, the expressed glucokinase was also co‐localized with actin filaments in the cytoplasm of these transfected cells. Although co‐localization of glucokinase with actin filaments was not clearly demonstrated in the pancreatic β‐cell line MIN6, islet glucokinase was found to be present in both the nucleus and the cytoplasm, though predominantly in the nucleus. These findings suggest that subcellular localization of glucokinase, including co‐localization with actin filaments, may have an important physiological role in metabolic regulation.