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Essential role of Rac GTPase in hydrogen peroxide‐induced activation of c‐fos serum response element
Author(s) -
Kim Jae-Hong,
Kwack Hee-Jin,
Choi Sung-E,
Kim Byung-Chul,
Kim Yong-Sun,
Kang Il-Jun,
Kumar Chandra C.
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00249-4
Subject(s) - serum response element , transfection , gtpase , phospholipase a2 , chemistry , small gtpase , reporter gene , signal transduction , microbiology and biotechnology , phospholipase c , hydrogen peroxide , serum response factor , mutant , inducer , rac gtp binding proteins , phospholipase , rac1 , biology , gene , biochemistry , enzyme , gene expression
In the present study, we investigated whether hydrogen peroxide activates c‐fos serum response element (SRE) in Rat‐2 fibroblast cells. By transient transfection analysis, exogenous H 2 O 2 stimulated SRE‐dependent reporter gene activity in a dose and time‐dependent manner. Also, we examined the role of Rac GTPase and phospholipase A 2 (PLA 2 ) in the H 2 O 2 ‐induced SRE activation. Either transfection of a dominant negative Rac mutant, RacN17, plasmid or pretreatment of mepacrine, a potent inhibitor of PLA 2 , blocked H 2 O 2 ‐induced SRE activation dramatically. Together, these findings suggest a critical role of ‘Rac and subsequent activation of phospholipase A 2 ’ in the signaling pathway of H 2 O 2 to SRE.