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Determination of DMPC hydration in the L α and L β′ phases by 2 H solid state NMR of D 2 O
Author(s) -
Faure Chrystel,
Bonakdar Lida,
Dufourc Erick J
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00201-9
Subject(s) - chemistry , deuterium , molecule , crystallography , solid state nuclear magnetic resonance , phase (matter) , solid state , nuclear magnetic resonance , physics , organic chemistry , quantum mechanics
The number of water molecules bound to the dimyristoylphosphatidylcholine (DMPC) interface was investigated both in the fluid (L α ) and gel (L β′ ) phases by solid state deuterium NMR of D 2 O. We determined that each DMPC molecule binds 9.7±0.5 and less than 4.3±0.5 D 2 O in the fluid and gel phases respectively. These results are accounted for by considering the number of DMPC binding sites as well as the molecular organization in each phase. © 1997 Federation of European Biochemical Societies.