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Oscillation of ADP‐ribosyl cyclase activity during the cell cycle and function of cyclic ADP‐ribose in a unicellular organism, Euglena gracilis
Author(s) -
Masuda Wataru,
Takenaka Shigeo,
Inageda Kiyoshi,
Nishina Hiroshi,
Takahashi Katsunobu,
Katada Toshiaki,
Tsuyama Shingo,
Inui Hiroshi,
Miyatake Kazutaka,
Nakano Yoshihisa
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00168-3
Subject(s) - euglena gracilis , euglena , cyclase , biochemistry , biology , cell division , cell cycle , dna synthesis , microbiology and biotechnology , cell , chemistry , enzyme , dna , chloroplast , gene
In Euglena gracilis , the activity of ADP‐ribosyl cyclase, which produces cyclic ADP‐ribose, oscillated during the cell cycle in a synchronous culture induced by a light‐dark cycle, and a marked increase in the activity was observed in the G2 phase. Similarly, the ADP‐ribosyl cyclase activity rose extremely immediately before cell division started, when synchronous cell division was induced by adding cobalamin (which is an essential growth factor and participates in DNA synthesis in this organism) to its deficient culture. Further, cADPR in these cells showed a maximum level immediately before cell division started. A dose‐dependent Ca 2+ release was observed when microsomes were incubated with cADPR. © 1997 Federation of European Biochemical Societies.