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Three different GB virus C/hepatitis G virus genotypes
Author(s) -
Mukaide Motokazu,
Mizokami Masashi,
Orito Etsuro,
Ohba Ken-ichi,
Nakano Tatsunori,
Ueda Ryuzo,
Hikiji Kazumasa,
Iino Shiro,
Shapiro Sarah,
Lahat Nitza,
Park Young-Min,
Kim Boo-Sung,
Oyunsuren Tsendsuren,
Rezieg Mohammed,
Al-Ahdal Mohammed N.,
Lau Johnson Y.N.
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00136-1
Subject(s) - gb virus c , genotyping , biology , phylogenetic tree , virology , genotype , restriction enzyme , polymerase chain reaction , virus , genetics , hepatitis c virus , flaviviridae , untranslated region , five prime untranslated region , gene , rna
The 5′‐untranslated region (5′‐UTR) sequences of 33 GB virus C/hepatitis G virus (GBV‐C/HGV) obtained from different geographic areas were determined through reverse‐transcription polymerase chain reaction and dideoxy chain termination sequencing, the alignment of sequences, the estimation of the number of nucleotide substitution per site, and construction of phylogenetic trees. The 5′‐UTR of GBV‐HGV was found to be heterogeneous, with 70.9–99.5% homology. Three distinct phylogenetic branches were observed consistently in all phylogenetic trees. GBV‐C is the prototype for one, HGV for another, and there is a new branch which consisted of GBV‐C/HGV isolates from Asia. Genotype‐specific restriction sites for the restriction enzymes, ScrFI and BsmFI , were identified, and a simple restriction fragment polymorphism analysis was developed for genotyping. These data provide evidence that GBV‐C/HGV consists of three different genotypes. Our simple genotyping assay will also provide a tool for epidemiological studies of GBV‐C/HGV infection.