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Regulation of maltose utilization in Saccharomyces cerevisiae by genes of the RAS/protein kinase A pathway 1
Author(s) -
Wanke Valeria,
Vavassori Monica,
Thevelein Johan M,
Tortora Paolo,
Vai Marco
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)00009-4
Subject(s) - saccharomyces cerevisiae , maltose , protein kinase a , biochemistry , chemistry , gene , kinase , microbiology and biotechnology , biology , enzyme
In Saccharomyces cerevisiae maltose utilization requires a functional MAL locus, each composed of three genes: MALR (gene 3) encoding a regulatory protein, MALT (gene 1) encoding maltose permease and MALS (gene 2) encoding maltase. We show that constitutive activation of the RAS/protein kinase A pathway severely reduces growth of MAL1 strains on maltose. This may be a consequence of reduction in MALT mRNA, reduced V max and increased catabolite inactivation of the MALT ‐encoded maltose transporter in the MAL1 strain. Mutations in the GGS1/TPS1 gene, which restricts glucose influx and possibly affects signalling, relieve carbon catabolite repression on both maltase and maltose permease and reduce maltose permease inactivation.