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Regulation of hyaluronate metabolism by progesterone in cultured fibroblasts from the human uterine cervix
Author(s) -
Tanaka Kanji,
Nakamura Toshiya,
Takagaki Keiichi,
Funahashi Masaru,
Saito Yoshiharu,
Endo Masahiko
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01538-4
Subject(s) - endocrinology , glucosamine , medicine , cervix , metabolism , hyaluronic acid , chemistry , hormone , biology , biochemistry , cancer , anatomy
The effects of progesterone, dehydroepiandrosterone sulfate and 17β‐estradiol on the synthesis and degradation of hyaluronate were investigated using human uterine cervix fibroblasts. The cells were incubated with [ 3 H]glucosamine in the presence of the hormones and then [ 3 H]hyaluronate was isolated from the medium. The changes in the radioactivity of [ 3 H]hyaluronate showed that progesterone suppressed hyaluronate synthesis by 22% of the control levels, while dehydroepiandrosterone sulfate and 17β‐estradiol enhanced it by 22% and 12% of the control levels, respectively. Furthermore, progesterone induced degradation of high‐molecular‐weight [ 14 C]hyaluronate into low‐molecular‐weight hyaluronate ( M r ≈40 000). These results suggest that in cultured fibroblasts from the human uterine cervix progesterone converts hyaluronate metabolism from the synthesis phase to the degradation phase. © 1997 Federation of European Biochemical Societies. All rights reserved