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Characterization of dominant lethal mutations in the yeast plasma membrane H + ‐ATPase gene
Author(s) -
Portillo Francisco
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01515-3
Subject(s) - mutant , allele , biology , gene , microbiology and biotechnology , yeast , wild type , genetics , atpase , mutation , phenotype , gene expression , saccharomyces cerevisiae , biochemistry , enzyme
Site‐directed mutants of yeast ATPase were studied after introduction of mutant alleles into a yeast strain where these alleles were constitutively expressed and the expression of the wild‐type chromosomal ATPase gene was turned off. One objection to this constitutive expression system was made apparent recently, as dominant lethal mutations are lost by gene conversion with the wild‐type allele during the process. Here, the phenotypes of the mutant alleles, which were studied in a constitutive expression system, are re‐evaluated under conditions in which these site‐directed mutants are conditionally expressed. We show that 12 of 25 site‐directed mutations previously described are actually dominant lethal alleles. In addition, we show that dominant mutant proteins interfere with transport of wild‐type ATPase to the plasma membrane.