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Identification of two new μ‐adaptin‐related proteins, μ‐ARP1 and μ‐ARP2
Author(s) -
Wang Xiaolu,
Kilimann Manfred W
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01500-1
Subject(s) - clathrin , complementary dna , signal transducing adaptor protein , biology , amino acid , microbiology and biotechnology , peptide sequence , cloning (programming) , computational biology , gene , biochemistry , endocytosis , receptor , computer science , programming language
We report the cDNA cloning, primary structure and tissue distribution of two new proteins homologous to μ‐adaptins, the medium chains of the clathrin coat adaptor complexes. Both predicted proteins share 60% amino acid sequence identity with each other and 27–31% identity with μ1‐adaptin (ap47) and μ2‐adaptin (ap50). Lower similarity (23–25% identity) is found with two other μ‐adaptin‐related proteins, p47A/B, and there is similarity over the N‐terminal 150 amino acids with the adaptin small chains and δ‐COP. The mRNAs of both molecules are expressed in all tissues analyzed, but with different profiles of relative abundance. μ‐ARP1 is most abundant in brain, ovary and lung, whereas μ‐ARP2 is prominently expressed in testis. These proteins suggest the existence of as yet uncharacterized types of clathrin‐ or non‐clathrin‐associated protein coats in cellular membrane traffic, of which they are probably prototype subunits, and provide molecular markers and probes for their characterization.