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Expression of the cell‐adhesion molecule VCAM‐1 by stromal cells is necessary for osteoclastogenesis
Author(s) -
Feuerbach Dominik,
Feyen Jean H.M
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01495-0
Subject(s) - stromal cell , vcam 1 , osteoclast , cell adhesion molecule , cell culture , cell adhesion , microbiology and biotechnology , chemistry , lymph node stromal cell , cell–cell interaction , cell , bone marrow , biology , icam 1 , immunology , cancer research , in vitro , biochemistry , genetics
Osteoblastic cells have been shown to be involved in osteoclast formation through cell to cell contacts. This study was designed to examine the possible function of vascular cell adhesion molecule 1 (VCAM‐1) during osteoclastogenesis. As a source for stromal cells we used the recently established mouse bone marrow stromal cell line mBMS‐B1 which has the ability to support osteoclastogenesis when used in co‐culture with a crude spleen cell suspension. mBMS‐B1 cells express a single ∼3.9 kb VCAM‐1 mRNA species. Expression was low under basal culture conditions and a 5–10‐fold increase was observed in the presence of 1,25(OH) 2 D 3 . Cell surface expression of VCAM‐1 examined by FACS analysis was increased about 2‐fold after 1,25(OH) 2 D 3 treatment. Immunoprecipitation of cell surface expressed VCAM‐1 or total VCAM‐1 protein using the anti‐VCAM‐1 monoclonal antibody MK2.7 resulted in a single ∼110 kDa protein on SDS‐PAGE. Induction by 1,25(OH) 2 D 3 was about 2–5‐fold on day 3. The stromal cell–osteoclast precursor cell interaction was investigated in a co‐culture of the mBMS‐B1 and mouse spleen cells in the presence of 1,25(OH) 2 D 3 . The monoclonal antibody MK2.7 which is known to block hemopoietic‐stromal cell recognition inhibited the formation of osteoclasts when added to the co‐culture at day 2 but not day 4. These data suggest that VCAM‐1 is involved in the interaction between stromal cells and osteoclastic precursor cells during osteoclastogenesis presumably most important during early stages of the formation of osteoclasts.