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C/EBPα is a major activator for the transcription of rat Cu/Zn superoxide dismutase gene in liver cell
Author(s) -
Kim Young Ho,
Yoo Hae Yong,
Chang Mun Seog,
Jung Guhung,
Rho Hyune Mo
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01487-1
Subject(s) - transactivation , microbiology and biotechnology , sod1 , superoxide dismutase , promoter , hepatocyte nuclear factors , transcription factor , activator (genetics) , hbx , gene , biology , chemistry , dismutase , gene expression , biochemistry , transfection , enzyme
The rat Cu/Zn superoxide dismutase (SOD1) is expressed in all tissues. Sequence analysis of the SOD1 promoter region showed that none of the cis ‐elements of hepatocyte‐specific nuclear factors (HNF) were observed. The cis ‐element of C/EBPα in the proximal region of the SOD1 promoter and the high level of C/EBPα in the liver tissue led us to focus on the transcriptional regulation of the SOD1 gene by C/EBPα. Cotransfection assays with the plasmid expressing transcription factor C/EBPα showed that C/EBPα transactivated SOD1 gene by 27 fold. The marked transactivation and direct binding of C/EBPα to the SOD1 promoter were confirmed by deletion analyses and mobility shift assays. These results suggested that C/EBPα plays a major role in the tissue distribution of SOD1.