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Nuclear translocation of PKCζ during ischemia and its inhibition by wortmannin, an inhibitor of phosphatidylinositol 3‐kinase
Author(s) -
Mizukami Yoichi,
Hirata Takao,
Yoshida Ken-ichi
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01481-0
Subject(s) - wortmannin , phosphatidylinositol , protein kinase c , diacylglycerol kinase , cytosol , chromosomal translocation , chemistry , kinase , microbiology and biotechnology , biochemistry , biology , enzyme , gene
Protein kinase Cζ (PKCζ), a member of the atypical PKC subgroup, is insensitive to Ca 2+ , diacylglycerol, and phorbol esters, but is activated by phospholipids such as phosphatidylinositol‐3,4,5‐triphosphate, a product of phosphatidylinositol 3‐kinase (PI3‐kinase). Here we show that PKCζ translocates from the cytosol to the 1000× g pellet (nuclear‐myofibrillar) fraction during ischemia for 40 min in Langendorff‐perfused rat hearts. In addition, immunohistochemical observation shows that ischemia induces the translocation of PKCζ to the nucleus. The nuclear translocation during ischemia is inhibited in a dose‐dependent manner by wortmannin (10 −9 –10 −7 M), an inhibitor of PI3‐kinase.