Premium
Murine tissue inhibitor of metalloproteinases—4 ( Timp —4): cDNA isolation and expression in adult mouse tissues 1
Author(s) -
Leco Kevin J.,
Apte Suneel S.,
Taniguchi Gary T.,
Hawkes Susan P.,
Khokha Rama,
Schultz Gilbert A.,
Edwards Dylan R.
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01474-3
Subject(s) - complementary dna , microbiology and biotechnology , tissue inhibitor of metalloproteinase , matrix metalloproteinase , biology , messenger rna , extracellular matrix , metalloproteinase , gene , biochemistry
We have isolated cDNA clones corresponding to a new member of the murine tissue inhibitor of metalloproteinase (TIMP) family, designated Timp ‐4. The nucleotide sequence predicts a protein of 22 609 Da that contains the characteristic 12 cysteine TIMP signature. TIMP‐4 is more closely related to TIMP‐2 and TIMP‐3 than to TIMP‐1 (48%, 45% and 38% identity, respectively). Analysis of Timp ‐4 mRNA expression in adult mouse tissues indicated a 1.2 kb transcript in brain, heart, ovary and skeletal muscle. This pattern of expression distinguishes Timp ‐4 from other Timp s, suggesting that the TIMP‐4 protein may be an important tissue‐specific regulator of extracellular matrix remodelling.