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Ozone depletes tocopherols and tocotrienols topically applied to murine skin
Author(s) -
Thiele Jens J,
Traber Maret G,
Podda Maurizio,
Tsang Kenneth,
Cross Carroll E,
Packer Lester
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(96)01463-9
Subject(s) - hairless , chemistry , vitamin e , malondialdehyde , ozone , polyethylene glycol , vitamin , peg ratio , tocopherol , antioxidant , biochemistry , pharmacology , organic chemistry , biology , finance , economics
To evaluate ozone damage to hairless mouse skin, two parameters of oxidative damage, vitamin E depletion and malondialdehyde (MDA) production, were measured in vitamin E‐enriched and in control skin from mice exposed to ozone (10 ppm). A 5% vitamin E solution (tocotrienol‐rich fraction, TRF) in polyethylene glycol (PEG) was applied to 2 sites on the back of hairless mice, PEG to 2 sites. After 2 h, the sites were washed, one of each pair of sites covered and the mice exposed ozone for 2 h. Ozone exposure (compared with covered sites) increased epidermal MDA in PEG‐treated sites, while vitamin E was unchanged. In contrast, ozone exposure significantly depleted vitamin E in TRF‐treated sites, while significant MDA accumulation was prevented. This is the first demonstration that ozone exposure causes damage to cutaneous lipids, an effect which can be attenuated by vitamin E application.